Activity of Exocrine Pancreatic Enzymes in Diabetic Female Rats

Abstract

The study designed to evaluate the activity of pancreatic exocrine enzymes in diabetic male rats induced by alloxan. The hyperglycemia was induced in forty-five rats after fasting of the animals for 24 hours by single intraperitoneal (i.p) injection of Alloxan 100 mg/kg B.W., three days after injection fasting blood glucose was measured when the concentration higher than 150 mg/dL, were considered as hyperglycemia/diabetes. A total of sixty adult male rats (45 diabetes and 15 non-diabetes) divided into two groups as follows. The first group serves as control groups (15 animals) will be single i.p injection with distilled water. the second group diabetic groups (45 animals from the first experiment) were subdivided into three subgroups as following (15 for each). Group (G1), Group (G2) and Group (G3) serve as 20, 40- and 60-days diabetic animals respectively. The blood samples collection were taken through cardiac puncture technique from each rat for each period days for measurement the following parameters: (Serum glucose, total protein, insulin, cholesterol, albumin, triglyceride, LDL-C, HDL-C, and VLDL-C) concentration, the rats pancreatic tissue were be taken for measured tissue pancreatic lipase, amylase, and trypsin concentration. The results demonstrate a significant increase in serum glucose concentration and a decrease in serum insulin and total protein in the diabetic group as compared with the control rats group in all experimental days. The results showed a significant rise in serum total cholesterol concentration within the diabetic group when compared with the control group at day 20 and 60. Meanwhile, a significant increase in serum triglyceride and LDL concentration and a significant decrease in serum HDL concentration within the diabetic group when compared with the control rats group at day 20, 40 and 60. But the serum VLDL concentration depicted a significant increase in the group of diabetic when compared with the control rats group at day 40 and 60.