This prospective study aims to identify and compare the incidence of bacterial contamination of hospital units and the distribution of species responsible for the contamination. The study has examined the level of bacterial contamination in these specialized care units in Al-Hussein Teaching Hospital. A total of 270 isolates have been collected and analyzed, 53.7% (n=145) were positive for bacterial growth and Pseudomonas aeruginosa approximately predominate in all the units sampled followed by Staphylococcus aureus 22% (n=32), Enterobacter spp. 20% (n=29), Escherichia coli 14% (n=20), Klebsiella pneumonia 11% (n=16), Acinetobacter spp. 3.4% (n=5), Citrobacter spp. 1.4% (n=2) and the least were Enterococcus spp. and Proteus spp. 0.7% (n=1). The relatively low level of bacterial contamination of the air compared to the high level with equipment and objects indicates the need for periodic microbiological surveillance aimed at early detection of bacterial contamination level.