Detection of Human Follicular Fluid Microorganisms at the Time of Oocyte Retrieval and its Impact on in-vitro Fertilization Outcomes

Samah Omar Noor,

Published on: 2020-08-03

Abstract

The follicular fluid (FF) one of the important sources of microorganisms that may affect in vitro fertilization (IVF) outcomes. The present study investigated the microorganisms within 50 FF of women undergoing IVF procedure. All samples were identified according to the morphological features, biochemical, and molecular methods. Twelve microbial species belonging to six genera were detected within 42% of FF samples and 58% did not show any microbial growth. The predominant species were Streptococcus spp. (34%), followed by Corynebacterium spp. (24%). Other microbes include Bacillus spp., Enterococcus spp., Staphylococcus spp., and Yeast. The FF microorganisms were close to statistically significant with IVF outcomes. Follicular fluid was not always sterile but contained a varied range of microorganisms that may effects the IVF outcomes and a larger sample of patients need to be studied to further examine our hypothesis.

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