Diagnosis of Giardia Species by Comparison of Two Enzyme-Linked Immuno-Sorbent Tests

Abstract

Background: There was some trade produced enzyme-immuno-sorbent assays tests to diagnose antigens of Giardia spin stool specimens, like Rida Quick Giardia and Serazym ELISA Giardia® tests. In this study, we evaluated the performance of two commercially available EIA kits for detecting Giardia antigens.
Methods: A total of 96 stool specimens were collected from patients who attended different Primary Health Care centers in Al-Qurna sector / Basrah Health Directorate, Ministry of Health/ Environment, Basrah, Iraq at period from 2nd of March 2018 to 28th of September 2018. All specimens were examined by conventional methods of microscopic examination, and by both enzyme-immuno-sorbent assays tests.
Results: Serazym ELISA Giardia® had a sensitivity of 90.1%, a specificity of 100%, and an accuracy of 91.7%. The Rida Quick Giardia® showed a sensitivity of 79%, a specificity of 100%, and an accuracy of 82.3%.SerazymELISA Giardia® showed 10% discrepancy better than Rida Quick Giardia® which showed 8%, this was of a statistically significant difference (P<0.05).
Conclusion: Antigen detection by EIA has been established as a valuable tool to make parasite stool diagnostics more effective. Serazym ELISA Giardia® is more reliable than Rida Quick Giardia®.