Evaluation of Serum and Follicular Fluid Levels of Advanced Glycation End products (AGEs) on ICSI Outcome

Abstract

Background: Advanced Glycation End products (AGEs) are produced by normal body physiology through non-enzymatic change of nucleic acid, protein, and lipid in hyperglycemic situation. However, thermal processing and modifications can lead to increased formation of AGEs, mainly long-term or high temperature cooking methods such as frying, roasting, and grilling. Elevated AGEs level in the body have an adverse effect on the general body health and females fertility, probably by a rise in oxidative stress and apoptosis.
Method: Sixty-six infertile females undergoing controlled ovarian hyperstimulation, their age range between 19-44 years old were included in this prospective comparative study. In the day of oocyte retrieval, collect follicular fluid and serum for subsequent measurement of AGEs by ELISA kits.
Result: Significant negative relationship was observed between the oocytes retrieved number, percentage of embryo G1, and cleavage rate with AGEs concentration in follicular fluid and serum. While significant positive correlation was observed between the numbers of GV oocytes (immature oocyte) with AGEs concentration in follicular fluid and serum.
Conclusion: There are negative effects of Advanced Glycation End products (AGEs) on the oocytes number and embryo G1, while there is a positive effect of AGEs on the number of GV oocytes.