Expression of P27 and P53 Protein in Hodgkin’s Lymphoma Patient Using Immunohistochemistry in Anbar Province

Abstract

Hodgkin’s lymphoma (HL) is the type of lymphoma, contains cells called Reed Sternberg cells. It is a common lymphoid malignancy that is diagnosed mostly in young adults. The present study was carried out on fifty Iraqi patients with Hodgkin’s lymphoma. They were visited AL-Ramadi Teaching hospital during the period from January 2016 to March 2017. The patients included 43 males and 7 females. Fifty blocks of tissue biopsy from those patients were collected. The diagnosis was achieved according to histopathological findings. Evaluation of p53 and p27 proteins in these biopsies was examined in paraffin-embedded tissue sections. The control group was ten slides from healthy individuals with no HL. Immunohistochemistry study was carried out on the tissue biopsy samples of those patients according to Ramos-Vera (2005) procedure. The results revealed that p53 overexpression was in 22 patients (44%) with (Score 3), 15 patients (30%) (Score 2), and finally 13 patients (26%) with (Score 4) while (score 0 and score 1) were characterized by no expression. Regarding to p27 protein expression of Hodgkin’s lymphoma patients, the results were 26 patients (52%) with (Score 3), 17 patients (34%) (Score 2), and finally 7 patients (14 %) with (Score 4) while absent in both (score 0 and score 1) .The presence of p53 and p27 proteins in the patients’ tissues with Hodgkin’s lymphoma indicates that expression of these genes and they are playing a role in the development and occurrence of these tumors.