Hydrophobicity and Adhesion Properties of Bacterial Growth Under Varying Temperature-Time Environment and Material Surface

Rita NN Rammo,

Published on: 2020-03-05


Hydrophobicity and adhesion of bacterial growth under varying temperature-time environment and material surface have been studied. Bacterial isolates were identified by standard methods of classification. Hydrophobicity index was assessed by microbial adhesion to hydrocarbon (MATH) utilizing absorbance at 520 nm; and adhesion by measuring crystal violet absorbance at 570 nm for various times (1,8,24 H), temperatures (5,25,40ºC) and material surfaces (PS,PP,PTFE). The results of P. aeruginosa and S. aureus showed a strong - moderate level of hydrophobicity at 40ºC in comparison to S. marcescens at a low level. In the adhesion assays to plastic materials, P. aeruginosa and S. aureus showed more adherence than S. marcescens and following the order as Teflon<polypropylene<polystyrene for all time-temperature range studied. Our results explain that the adhesion mechanism is governed by the triad interaction bacteria environment material surface, each of which contributes its attribute that when optimum conditions are favorite lead up to infection. 

scroll up