In-vitro Evaluation of the Antimicrobial Activities of Chitosan and Chitosan-PVP Linked Nanocomposite Films against Some Multidrug Resistant Bacteria

Abstract

Many polymeric materials are able to kill multidrug-resistant bacteria without inducing drug resistance or toxic side effects on the patient. A variety of antibacterial polymeric nano systems have exhibited great inhibition of multidrug resistant bacteria, prevent biofilm formation, enhance bacterial recognition and binding capabilities. The present study describes the effect of incorporating between two polymers; chitosan and polyvinyl pyrrolidone with CuO, TiO2 and Ag nanoparticles on some bacterial pathogens. The antimicrobial activity of the prepared polymers was determined by four methods; diffusion method, colony forming unit (CFU), Optical density and anti adhesive method. The plants beetroot was extracted by boiling water to obtain the extract. The results showed that the nanocomposite film was active against different pathogenic bacteria. In Gram negative Pseudomonas aeruginosa was the most sensitive to incorporating film. In Gram positive MRSA was effective with nanocomposite film. The blends of chitosan, PVP and Nanoparticles (CuO, TiO2 , and Ag) were good antibacterial ability on gram positive and negative bacteria.