Investigating the Effect of Beta-Defensin 126 Gene on Infertility Treatment by Intrauterine Insemination of Sperm through Genetic Analysis

Abstract

This study aimed to determine the association between the mutation of Human β-defensin 126 (DEFB126) and the rate of success of intrauterine insemination (IUI) of sperm. In this study, DEFB126 gene mutation was detected in 76 Iranian males with infertility reasons for which women had positive IUI and negative IUI. After the extraction of DNA from the blood of the patients, the DNA quality extracted by the spectrophotometer was evaluated. Methods (PCR-SSCP) and sequencing were also used to confirm the results. Also, an immunocytochemistry technique was used to determine the expression of this protein on the surface of sperm cells. The results of the DNA review showed that 24.4% of the men whose fertility results were negative for their spouses had mutations, while those whose fertility results were positive did not have this mutation. Also, there was a lower protein content on the sperm level of men with a mutated allele. The results of this study indicate that having a mutant homozygote allele in the beta-daphnin 126 sequence is an effective factor in the failure of treatment with IUI.