Relation between the Angiotensin II Type 1 Receptor (AGTR1)-521C/T Gene Polymorphism and Blood Pressure

Alhashemi Wisam Kadhum H ,

Published on: 2020-02-10

Abstract

Angiotensin II sort one (AT1) receptor mediates the constriction and growth-promoting result of angiotensin in humans. It has been reported that a polymorphism of the AT1 receptor gene (a C/T at position-521) The single nucleotide polymorphism (SNP) -521C/T is located within the promoter region of the agtr1 gene, may be associated with essential hypertension (HT). The objective of this study is the analysis of C-521T polymorphism in angiotensin II type 1 receptor (AGR1) in Baghdad patients with essential hypertension as well as controls. We examined 50 patients with essential hypertension and 50 normotensive patients. In order to identify the C-521T angiotensin II type 1 receptor variant, we used the following methods: DNA extraction, PCR amplification and enzymatic digestion of the PCR product using Ssp I restriction endonuclease enzyme and the PCR product sent to Macrogene Company for DNA sequencing. In the study groups, the -521C/T variant was found more frequently in control subjects (68%), than in hypertensive patients (60%). We identified (30%) heterozygotes in the hypertensive group compared with (20%) in the control group and (27%) homozygotes in the hypertensive group compared with (24%) in the control group. No association was detected between angiotensin II type 1 receptor (AGTR1)-521 C/T gene polymorphism and blood pressure.

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