Role of p16 INK4A Protein in Evaluation of Benign, Premalignant and Malignant Cervical Lesion in Iraqi Patients (Immunohistochemical Study)

Alaa Salah Jumaah,

Published on: 2020-06-08

Abstract

Aim: Present study designed to evaluated immunohistochemical expression of both p16 INK4a and HPV (Human papillomavirus). Different cervical lesion (Benning, Premalignant and malignant) and its relation one to other in development of cervical carcinoma.
Materials and Methods: forty-nine cases of cervical lesion formalin fixed - paraffin embedded tissue specimens of the cases. Stained tissue sections of all the cases were evaluated to confirm diagnosis and assure the presence of representative tissue material. All cases with tissue sections that were appropriate for immunohistochemical evaluation, positive tissue controls were obtained according to primary antibodies manufacturer’s data-sheets tissue sections of a uterus adenosquamous as p16 primary antibody, sections of skin wart tissue were used as a positive control for HPV marker. Statistical analysis was performed between the immunohistochemistry for marker each other.
Results: The test results for control group: All patients had with histopathology changes and staining of cytoplasm. Among Benign group; twenty samples (100%) showed benign lesion change. Among premalignant group seven samples (78%) showed CIN-1 change. two samples (22%) showed CIN-2. Among Malignant group; six samples (30%) showed poorly differentiated. Eleven samples (55%) showed moderate differentiated. three samples (15%) showed well differentiated. Also, the intensity of premalignant lesion shows between weak and moderate and no one showing in strong, while for malignant intensity showing two sample in weak and two sample in moderate and two strong intensity.

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