Objective: This study was aimed to investigate the changes in some immunological markers with different concentrations of vitamin D. The parameters are immunoglobulin (IgA, IgM, and IgG,), anti-phospholipid ( IgM, IgG), Anti-cardiolipin ( IgM, IgG) and Erythrocyte Sedimentation Rate (ESR).
Methods: The specimens were collected from 90 volunteers. The samples were separated into three groups. The first group includes specimens with a normal value of vitamin D and the second group includes, people with marginal deficiency levels, as well as, the group with vitamin D deficiency. These tests were done on samples of ninety single males and their ages were 20-25 years. Serum Immunoglobulin (IgA, IgM, IgG) is detected by using Single Radial Immuno Diffusion (SRID). Enzymelinked Immune Sorbent Assay (ELISA) technique was used to measure the concentration of vitamin D, anti-phospholipid (IgM, IgG) and anti-cardiolipin( IgM, IgG). Westergren method was used to measure Erythrocyte Sedimentation Rate (ESR).
Results: There was a change in levels of immunological indicators involved in this study. The differences were significantly decreased (P<0.05) in the case of IgA concentration but it was non-significant in the case of IgM, IgG. The present study showed that the APL (IgM, IgG) and ACL (IgM, IgG) slightly increased but it did not reach the level of significance. The level of ESR increased significantly (P <0.05).
Conclusions: Changes in immunological processes for many parameters that shared in the current study was associated with both marginal deficiency group and vitamin D deficiency group. Vitamin D deficiency also activates the inflammatory cells to increase and decrease of inflammatory indicators.